A Method for Determination of Lactose in Milk by Paper Chromatography

Abstract

A quantitative method for lactose determination in milk by chromatographic analysis is described. Direct application of milk to the strip and subsequent determination of lactose on the chromatostrip after partitioning are desirable features of the procedure. The reproducibility of the method was determined for lactose in pure solution and in milk. At a mean value of 4.516% lactose, anhydrous, in milk the optimum standard deviation was 0.16%. The recovery from milk of added lactose, anhydrous, varied between 92 and 97% and averaged 95%. © 1953, American Dairy Science Association. All rights reserved.