Abstract
Summary: We developed a CGI/Perl-based web server to perform in silico polymerase chain reaction (PCR) on PCR primer sequences. The PUNS (Primer-UniGene Selectivity) server simulates PCR reactions by running BLASTN analysis on user-entered primer pairs against both the transcriptome and the genome to assess primer specificity. PUNS is particularly suited for the identification of highly selective primers for quantitative microarray validation. © Oxford University Press 2004; all rights reserved.
Cite
CITATION STYLE
Boutros, P. C., & Okey, A. B. (2004). PUNS: Transcriptomic- and genomic-in silico PCR for enhanced primer design. Bioinformatics, 20(15), 2399–2400. https://doi.org/10.1093/bioinformatics/bth257
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