Chimeric compounds combine the structural features of inhibitors of histone deacetylases (HDACi) and tyrosine kinase inhibitors (TKi), and therefore unite the effects of a dual-targeting strategy in one compound. Here, we describe the generation of such hybrid molecules. Small molecules, known as TKi, are combined with a Zn2+ chelating motive, preferentially a hydroxamic acid, in addition. The resulting small molecules also can inhibit histone deacetylases, which are dependent on the catalytically active Zn2+. Moreover, we summarize how the growth-inhibitory effects of these combined compounds can be determined with a simple proliferation assay with a leukemic cell line.
CITATION STYLE
Mahboobi, S., Pilsl, B., & Sellmer, A. (2017). Generation and assessment of fusions between HDACi and TKi. In Methods in Molecular Biology (Vol. 1510, pp. 405–412). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6527-4_31
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