Recent advances in global translatome analysis technologies enable us to understand how translational regulation of gene expression modulates cellular functions. In this chapter, we present an integrated method to measure various aspects of translatome by polysome profiling and ribosome profiling using purified B cells. We standardized our protocols to directly compare the results from these two approaches. Parallel assessment of translatome with these two approaches can generate a comprehensive picture on how translational regulation determines protein output.
CITATION STYLE
Jin, H. Y., & Xiao, C. (2018). An integrated polysome profiling and ribosome profiling method to investigate in vivo translatome. In Methods in Molecular Biology (Vol. 1712, pp. 1–18). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7514-3_1
Mendeley helps you to discover research relevant for your work.