Abstract
PEG-chitosan-PEI copolymer was synthesized by ring-opening reaction of ethylenimine monomers with PEGchitosan. PEG-chitosan was prepared before by an imine reaction between chitosan and mPEG-CHO. With the aim to investigate the gene transfection properties of PEG-chitosan-PEI, we investigated its cytotoxicity by MTT assay, DNA compaction ability by agarose gel retardation, and transfection efficiency of pEGFP in vitro. The results showed higher transfection efficiency and lower cytotoxicity of PEG-chitosan-PEI than PEI(25k) which is a popular gene vector. © 2008 Springer-Verlag.
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Zhang, W., Pan, S. R., Wen, Y. T., & Luo, X. (2008). Synthesis and gene transfection efficiency of PEG-chitosan-PEI copolymers. In IFMBE Proceedings (Vol. 19 IFMBE, pp. 9–12). Springer Verlag. https://doi.org/10.1007/978-3-540-79039-6_3
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