Live fluorescent microscopy of whole-mount rodent retinal explants has proved to be extremely valuable for understanding dynamic events during retinogenesis. However, to obtain three-dimensional images with high-quality axial resolution, investigators are restricted to specific areas of the retina and require microscopes, such as two photon, with a higher level of depth penetrance. As an alternative, we report a retinal live-imaging protocol using slice cultures that are suitable for capturing discrete cellular events during retinal development and differentiation. This is a significant improvement upon current methods, as it is more amenable to a wider array of imaging systems and does not compromise resolution of retinal cross-sectional area.
CITATION STYLE
Barrasso, A. P., & Poché, R. A. (2020). Live Imaging of Mouse Retinal Slices. In Methods in Molecular Biology (Vol. 2092, pp. 45–53). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0175-4_4
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