Angiogenesis, the growth of new blood vessels, is crucial for efficient skeletal muscle regeneration. Myogenesis and angiogenesis take place concomitantly during muscle regeneration. Myogenic precursor cells (MPCs) are in close proximity to vessels and interact with neighboring endothelial cells (ECs) to expand and differentiate. To demonstrate functional interplay between the two cell types, we established a robust and predictive ex vivo assay to evaluate activity of MPCs on angiogenesis and vice-et-versa, of ECs on myogenesis. Here, we describe an optimized three-dimensional co-culture protocol for the assessment of biological interactions between MPCs and ECs during skeletal muscle regeneration.
CITATION STYLE
Latroche, C., Weiss-Gayet, M., & Chazaud, B. (2019). Investigating the vascular niche: Three-dimensional co-culture of human skeletal muscle stem cells and endothelial cells. In Methods in Molecular Biology (Vol. 2002, pp. 121–128). Humana Press Inc. https://doi.org/10.1007/7651_2018_182
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