Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR + T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8+ and CD4+ T lymphocytes for in vitro and in vivo characterization.
CITATION STYLE
John, L. B., Chee, T. M., Gilham, D. E., & Darcy, P. K. (2014). Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor. Methods in Molecular Biology, 1139, 177–187. https://doi.org/10.1007/978-1-4939-0345-0_16
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