Cell-free protein synthesis (CFPS) enables rapid protein expression for the structural and functional characterization of proteins. Implementation of CFPS in a microfluidic platform has additional benefits such as reduced reaction volumes and simultaneous expression of multiple proteins. Here, we describe a microfluidic device that is composed of 96 continuous-exchange cell-free protein expression units and produces a protein synthesis yield up to 87 times higher than a conventional batch system. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Jackson, K., Khnouf, R., & Fan, Z. H. (2014). Cell-free protein synthesis in microfluidic 96-well plates. Methods in Molecular Biology, 1118, 157–168. https://doi.org/10.1007/978-1-62703-782-2_10
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