Single-cell bisulfite sequencing (scBS-seq) enables profiling of DNA methylation at single-nucleotide resolution and across all genomic features. It can explore methylation differences between cells in mixed cell populations and profile methylation in very rare cell types, such as mammalian oocytes and cells from early embryos. Here, we outline the scBS-seq protocol in a 96-well plate format applicable to studies of moderate throughput.
CITATION STYLE
Galvão, A., & Kelsey, G. (2021). Profiling DNA methylation genome-wide in single cells. In Methods in Molecular Biology (Vol. 2214, pp. 221–240). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0958-3_15
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