Application of DNA Fingerprinting in the Hokkaido Brown Bear (Ursus arctos yesoensis)

Abstract

DNA fingerprinting employing a minisatellite Myo probe was used for individual identification and paternity determination in Hokkaido brown bears (Ursus arctos yesoensis). We used two restriction enzymes, Hinfl and Haelll to make DNA fingerprints. Band patterns obtained from randomly selected bears were compared with each other, and the probability x that fragment in an individual was also present in the other was 0.69 for Hinfl and 0.83 for Haelll. The value for Hinfl (0.69) was similar to that obtained from other species, such as dog and domestic animals, and the mean probability of all fragments was calculated to be 2.5 x 10-2. The results suggest that DNA fingerprinting applying the combination of Hinfl and Myo is available for individual identification. On the other hand, the ability to determine paternity seemed to be insufficient owing to the lack of paternal fragments, although the band patterns reflected the correct relationships between child and father. © 1994, JAPANESE SOCIETY OF VETERINARY SCIENCE. All rights reserved.