The journey to in vivo synthetic chemistry: From azaelectrocyclization to artificial metalloenzymes

Abstract

The goal of this account is to detail the steps taken by our group for the development of glycosylated artificial metalloenzymes (GArMs), which we have used in our endeavors to develop examples of in vivo synthetic chemistry. To accomplish this, we have had to combine technologies developed over the course of a decade that range from protein ligation methodologies, identification of glycan-dependent targeting modules, and the development of functional biocatalysts. As an end result, we have begun to show the early framework for GArM complexes and their potential towards creating novel biotechnological tools and therapeutic applications.