Effect of Edible Oil on AMP-Activated Protein Kinase Pathway in Rabbit

Abstract

AMP activated protein kinase (AMPK) regulates various signalling pathways which are concerned with upholding energy equilibrium. In many catabolic and anabolic pathways, AMPK acts as a switch control. The present study is to find out the effect of edible oil on AMPK pathway in rabbit lipid Metabolism. The study is conducted as per CPCSEA guidelines by dividing the animals into three batches namely control, standard and unknown for 21 days fed with different doses of edible oil and termed as batch I, batch II and batch III respectively. Liver tissue is extracted from the animals and is processed for the protein estimation studies using Bradford assay, Western blot, and biochemical analysis of anitoxidants namely superoxide dismutase (SOD), reduced glutathione (GSH) and Glutathione Peroxidase (GPx), by ELISA method. The above all parameters were comparatively correlated with AMPK gene expression by Real Time PCR. GSH activity in control liver tissue and oil fed liver tissue, the levels of GSH in the Batches II and III were higher compared to the control Batch I. There was also statistical significance among the Batches. Unlike GSH, GPx showed its results in contrast with GSH. SOD activity has not shown any significance difference. By ELISA, it is found that AMPKα2 levels are enhanced by long term oil feeding. The role of AMPK in glycolytic flux up-regulation and contribution for antioxidant production that is required for liver survival through GSH is illustrated. However, continued down regulation of AMPK leads to oxidative stress and metabolic crisis, which in turn leads to cell death. Therefore, the present study, which is first of its kind, throw insights to understand better the responses against oxidative stress of rabbit liver and unravels the molecular basis of the patho-physiology of diseases like metabolic syndrome, cancer, obesity, type II diabetes and longevity.