Analyzing individual cells allows detecting a minor group of abnormal cells present in a large population of normal cells. This ability can be essential to understanding diseases, such as cancer and diabetes. Microchip electrophoresis (MCE) is the technique of choice for single-cell analysis. However, since the channels in microfluidic devices are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe an MCE method with highly sensitive chemiluminescence detection for simultaneous determination of multiple amino acids present in single cells.
CITATION STYLE
Liu, Y. M., & Zhao, S. (2012). Quantification of amino acids in a single cell by microchip electrophoresis with chemiluminescence detection. Methods in Molecular Biology, 828, 351–358. https://doi.org/10.1007/978-1-61779-445-2_28
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