The caspase-1 enzymatic activity plays a major role in the innate immune response as it regulates the maturation of two major proinflammatory cytokines, the interleukin-1beta (IL-1β) and IL-18. In this chapter, we describe the technique of Western blot to assess caspase-1 activation. This method provides multiple information within one experiment. It allows the detection of both unprocessed and processed caspase-1 and substrates.
CITATION STYLE
Guey, B., & Petrilli, V. (2016). Assessing caspase-1 activation. In Methods in Molecular Biology (Vol. 1417, pp. 197–206). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3566-6_13
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