The Single Cell Gel Electrophoresis (Comet) assay is a simple, versatile and sensitive method for measuring DNA damage in individual cells, allowing the determination of heterogeneity of response within a cell population. The basic alkaline technique described is for the determination of DNA strand break damage and its repair at a single cell level. Specific modifications to the method use a lower pH ('neutral' assay), or allow the measurement of DNA interstrand cross-links. It can be further adapted to, for example, study specific DNA repair mechanisms, be combined with fluorescent in situ hybridisation, or incorporate lesion specific enzymes.
CITATION STYLE
Hartley, J. M., Spanswick, V. J., & Hartley, J. A. (2011). Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay (pp. 309–320). https://doi.org/10.1007/978-1-61779-080-5_25
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