Why does Escherichia coli have two primary pathways for synthesis of glutamate?

97Citations
Citations of this article
90Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Escherichia coli has two primary pathways for glutamate synthesis. The glutamine synthetase-glutamate synthase pathway is known to be essential for synthesis at low ammonium concentrations and for regulation of the glutamine pool, but the necessity for glutamate dehydrogenase (GDH) has been uncertain. The results of competition experiments between the wild type and a GDH- deficient mutant during nutrient-limited growth and of direct enzyme measurements suggest that GDH is used in glutamate synthesis when the cell is limited for energy (and carbon) but ammonium and phosphate are present in excess, while the glutamine synthetase-glutamate synthase pathway is used when the cell is not under energy limitation. The use of alternative routes for glutamate synthesis implies that the energy cost of biosynthesis may be less when energy is limited than when energy is unlimited.

Cite

CITATION STYLE

APA

Helling, R. B. (1994). Why does Escherichia coli have two primary pathways for synthesis of glutamate? Journal of Bacteriology, 176(15), 4664–4668. https://doi.org/10.1128/jb.176.15.4664-4668.1994

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free