Validation of a combined enzymatic and HPLC method for screening of pectins in fruits of Japanese quince (Chaenomeles japonica)

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Abstract

A combined enzymatic and HPLC method was validated for analysis of galacturonic acid (GalA) in (1) alcohol-insoluble solids (AIS), and in (2) pectins, extracted from fruits of Japanese quince (Chaenomeles japonica), by comparison with a standard colorimetric method (using metahydroxydiphenyl) for analysis of uronic acids (UA). For analysis of AIS, the results of the colorimetric and the enzymatic/HPLC methods were identical (UA and GalA in average 20 g/100 g AIS dry weight). For analysis of pectins, the colorhnetric method resulted in consistently higher estimates (UA in average 63 g/100 g pectins dry weight) compared to the enzymatic/HPLC method (GalA in average 57 g/100 g pectins dry weight). The enzymatic/HPLC method also proved to be useful as a simple and efficient screening method, since a very high Pearson correlation (R=0.93) was obtained between GalA estimates of the homogenized fruit sample and the AIS content of acid-extracted pectins. © 2002 Elsevier Science Ltd. All rights reserved.

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Rumpunen, K., Thomas, M., Badilas, N., & Thibault, J. F. (2002). Validation of a combined enzymatic and HPLC method for screening of pectins in fruits of Japanese quince (Chaenomeles japonica). LWT, 35(6), 490–496. https://doi.org/10.1006/fstl.2002.0895

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