Spore production of entomopathogenic fungus Beauveria bassiana BNBCRC for biocontrol: Response surface optimization of medium using decanter cake from palm oil mill

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Abstract

Beauveria bassiana is an efficient entomopathogenic fungus for biological control. Optimization of medium composition for production of B. bassiana BNBCRC spores in decanter cake (DC)-based solid culture was performed using response surface methodology (RSM). Effects of inorganic and organic nitrogen sources, mineral salt, and incubation temperature in a DC-based composition were experimentally studied in two stages, first using a Taguchi design. Among the decision variables tested, urea, peptone, and MgSO4 significantly affected the spore concentration, and the maximal 1.89×108 spores g-1 was found at 30°C incubation temperature. In the second stage, these three selected decision variables were optimized by RSM, using a factorial central composite design. The maximum average spore concentration of B. bassiana BNBCRC increased to 4.80×108 spores g-1 in the optimal DC-based medium containing 0.80 g L-1 urea, 2.10 g L-1 peptone, and 0.75 g L-1 MgSO4. The coefficient of determination was R2=0.97 for the RSM model fit to data. Compared to the original DC-based medium, the performed optimization increased the spore production of B. bassiana BNBCRC by 2.5 fold. After optimization of moisture content, the DC -based medium containing 60% moisture gave the highest spore concentration of 4.71×108 spores g-1. © 2014 The Korean Society for Applied Biological Chemistry.

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Petlamul, W., & Prasertsan, P. (2014). Spore production of entomopathogenic fungus Beauveria bassiana BNBCRC for biocontrol: Response surface optimization of medium using decanter cake from palm oil mill. Journal of the Korean Society for Applied Biological Chemistry, 57(2), 201–208. https://doi.org/10.1007/s13765-013-4175-5

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