Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.
CITATION STYLE
Shashkova, S., Nyström, T., & Leake, M. C. (2022). Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells. In Methods in Molecular Biology (Vol. 2476, pp. 183–190). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2221-6_14
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