A transcriptomal analysis of bovine oviductal epithelial cells collected during the follicular phase versus the luteal phase of the estrous cycle

58Citations
Citations of this article
63Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Background: Reproductive success depends on a functional oviduct for gamete storage, maturation, fertilization, and early embryonic development. The ovarian-derived steroids estrogen and progesterone are key regulators of oviductal function. The objective of this study was to investigate luteal and follicular phase-specific oviductal epithelial cell function by using microarray-based transcriptional profiling, to increase our understanding of mRNAs regulating epithelial cell processes, and to identify novel genes and biochemical pathways that may be found to affect fertility in the future. Methods: Six normally cycling Angus heifers were assigned to either luteal phase (LP, n = 3) or follicular phase (FP, n = 3) treatment groups. Heifers in the LP group were killed between day 11 and 12 after estrus. Heifers in the FP group were treated with 25mg PGF 2aα (Lutalyse, Pfizer, NY) at 8pm on day 6 after estrus and killed 36h later. Transcriptional profiling by microarray and confirmation of selected mRNAs by real-time RT-PCR analyses was performed using total RNA from epithelial cells isolated from sections of the ampulla and isthmus collected from LP and FP treatment groups. Differentially expressed genes were subjected to gene ontology classification and bioinformatic pathway analyses. Results: Statistical one-way ANOVA using Benjamini-hochberg multiple testing correction for false discovery rate (FDR) and pairwise comparison of epithelial cells in the ampulla of FP versus LP groups revealed 972 and 597 transcripts up- and down-regulated, respectively (P < 0.05). Within epithelial cells of the isthmus in FP versus LP groups, 946 and 817 transcripts were up- and down-regulated, respectively (P < 0.05). Up-regulated genes from both ampulla and isthmus were found to be largely involved in cholesterol biosynthesis and cell cycle pathways, while down-regulated genes were found in numerous inflammatory response pathways. Conclusions: Microarray-based transcriptional profiling revealed phase of the cycle-dependent changes in the expression of mRNA within the epithelium of the oviducts' ampulla and isthmus.

References Powered by Scopus

Analysis of relative gene expression data using real-time quantitative PCR and the 2<sup>-ΔΔC</sup>T method

150298Citations
N/AReaders
Get full text

Gene Expression Omnibus: NCBI gene expression and hybridization array data repository

10066Citations
N/AReaders
Get full text

Exploration, normalization, and summaries of high density oligonucleotide array probe level data.

8787Citations
N/AReaders
Get full text

Cited by Powered by Scopus

An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

111Citations
N/AReaders
Get full text

Oviductal extracellular vesicles interact with the spermatozoon’s head and mid-piece and improves its motility and fertilizing ability in the domestic cat

79Citations
N/AReaders
Get full text

The oviduct: From sperm selection to the epigenetic landscape of the embryo

69Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Cerny, K. L., Garrett, E., Walton, A. J., Anderson, L. H., & Bridges, P. J. (2015). A transcriptomal analysis of bovine oviductal epithelial cells collected during the follicular phase versus the luteal phase of the estrous cycle. Reproductive Biology and Endocrinology, 13(1). https://doi.org/10.1186/s12958-015-0077-1

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 28

67%

Researcher 8

19%

Professor / Associate Prof. 4

10%

Lecturer / Post doc 2

5%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 21

49%

Biochemistry, Genetics and Molecular Bi... 9

21%

Medicine and Dentistry 7

16%

Veterinary Science and Veterinary Medic... 6

14%

Save time finding and organizing research with Mendeley

Sign up for free