Some Phytochemicals and Related Compounds in Vegetables as Potent Inhibitors of Human DNA Topoisomerase II

Abstract

An established human DNA topoisomerase II inhibitory assay is described, and the screening results for some phytochems. in vegetables and related compds. that may useful for chemoprevention through food are reported. The assay requires a small amt. of sample and is rapid compared to bioassays using cell lines or animals. The topoisomerase II inhibitory assay is useful for screening compds. in vegetables. More than 80 compds. were screened, and 28 compds. were potent inhibitors at the 10 μg/mL level. Many of the phytochems. commonly found in vegetables are thus useful for chemoprevention. [on SciFinder(R)]