In vivo tracking of particulate antigen localization and recognition by B lymphocytes at lymph nodes

Abstract

The development of experimental systems that allow in vivo antigen tracking as well as the study of B cell dynamics in real time and in situ, have transformed our understanding of the “how, when and where” B lymphocytes find antigen at secondary lymphoid organs in the last 10 years. Here, I described one of these experimental models, which uses highly fluorescent particulate antigen and B cell receptor (BCR)-transgenic B cells labeled with long-term fluorescent probes, combined with confocal and multiphoton microscopy.