Oat-maize addition lines, with individual maize (Zea mays L.) chromosomes added to the oat (Avena sativa L.) genome via wide hybridization and embryo rescue, simplify the maize genome by 10-fold. Radiation hybrids, derived through gamma irradiation of monosomic addition lines, have less than a complete maize chromosome in an oat genomic background. Maize genes and gene families can be readily located to their respective physical chromosome or chromosome segment by rapid, high-throughput, and straight-forward PCR experiments. Polymorphisms are not required to map a maize DNA fragment with this system. Numerous non-mapping uses of these lines currently underway are described. Seed and/or DNA of the addition lines in various oat and maize backgrounds are available as well as approximately 650 radiation hybrid lines.
CITATION STYLE
Phillips, R. L., & Rines, H. W. (2009). Genetic analyses with oat-maize addition and radiation hybrid lines. In Handbook of Maize: Genetics and Genomics (pp. 523–538). Springer New York. https://doi.org/10.1007/978-0-387-77863-1_26
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