Detection of Wolbachia in a thelytokous population of Trichogramma atopovirilia Oatman & Platner (Hymenoptera: Trichogrammatidae) using PCR with the wsp specific primer

Abstract

For the first time in Brazil, Wolbachia was detected in Trichogramma using PCR with the wsp specific primer. A Trichogramma atopovirilia Oatman & Platner population was colected in Helicoverpa zea Boddie (Lepidoptera: Noctuidae) eggs at Embrapa/Sete Lagoas. The results showed that the amplification of DNA bands, confirms the presence of Wolbachia on the analysed population. The presence of this α-proteobacteria will contribute for the correct choice on which population will be used for applied biological control programs against lepidopterous pests.