Histochemical staining and light microscopy-based techniques have been widely used to detect and quantify arbuscular mycorrhizal fungi (AMF) in roots. Here we describe a standardized method for staining of AMF in colonized roots, and we provide possible modifications to adjust the protocol according to particular requirements, such as the type of root material or the reduction of toxic products. In addition, we also summarize some of the most common ways to quantify arbuscular mycorrhizal colonization.
CITATION STYLE
Ho-Plágaro, T., Tamayo-Navarrete, M. I., & García-Garrido, J. M. (2020). Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization. In Methods in Molecular Biology (Vol. 2146, pp. 43–52). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0603-2_4
Mendeley helps you to discover research relevant for your work.