This study reports the introduction of gfp marker in two endophytic bacterial strains (Pantoea agglomerans C33.1, isolated from cocoa, and Enterobacter cloacae PR2/7, isolated from citrus) to monitor the colonization in Madagascar perinwinkle (Catharanthus roseus). Stability of the plasmid encoding gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under non-selective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated perinwinkle plants that grew for 10 and 20 days. Gfp-expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the P. agglomerans C33:1gfp in plants 20 days after inoculation, even in the absence of selective pressure, suggests that is good colonizer. These results indicated that both gfp-tagged strains, especially P. agglomerans C33.1, may be useful tools to deliver enzymes or other proteins in plant. © 2013 Springer-Verlag Berlin Heidelberg.
CITATION STYLE
Torres, A. R., Araújo, W. L., Cursino, L., De Barros Rossetto, P., Mondin, M., Hungria, M., & Azevedo, J. L. (2013). Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker. Archives of Microbiology, 195(7), 483–489. https://doi.org/10.1007/s00203-013-0897-3
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