A method for obtaining large populations of synchronized caenorhabditis elegans dauer larvae

Abstract

The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purifi ed dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals.