Oxidative stress responses of some endemic plants to high altitudes by intensifying antioxidants and secondary metabolites content

Abstract

Most endemic plant species have limited altitudinal ranges. At higher altitudes, they are subjected to various environmental stresses. However, these plants use unique defense mechanisms at high altitudes as a convenient survival strategy. The changes in antioxidant defense system and accumulation of different secondary metabolites (SMs) were investigated as depending on altitude in five endemic endangered species (Nepeta septemcrenata, Origanum syriacum subsp. Sinaicum, Phlomis aurea, Rosa arabica, and Silene schimperiana) naturally growing in Saint Katherine protectorate (SKP). Leaves were collected from different sites between 1600 and 2200 m above sea level to assess the biochemical and physiological variations in response to high altitudes. At higher altitudes, the soil pH and micronutrient soil content decreased, which can be attributed to lower mineralization processes at lower pH. Total phenols, ascorbic acid, proline, flavonoids, and tannins increased in response to different altitudes. SMs progressively increased in the studied species, associated with a significant decrease in the levels of antioxidant enzyme activity. R. arabica, as the most threatened plant, showed the maximum response compared with other species. There was an increase in photosynthetic pigments, which was attained via the increase in chlorophyll a, chlorophyll b, and carotenoid contents. There was a significant increase in total soluble sugars and total soluble protein content in response to different altitudes. SDS-PAGE of leaf proteins showed alteration in the protein profile between different species and the same species grown at a different altitude. These five species can adapt to high-altitude habitats by various physiological mechanisms, which can provide a theoretical basis for the future conservation of these endangered endemic species in SKP.