Assessing genetic diversity in 23 early Polish oat cultivars based on molecular and morphological studies

Abstract

In Poland oat breeding began at the late nineteenth century. During the World War II almost all of Polish breeding materials were lost, and then were replaced by German cultivars. The main aim of this paper was to show the level of genetic diversity of Polish oat cultivars which were bred before 1939. Simultaneously usefulness and informativeness of molecular and morphological methods were tested and compared. This study involved 23 cultivars, which were described by 25 morphological traits and three types of molecular markers (AFLP, ISSR and RAPD). Based on Dice coefficient, genetic distance between cultivars ranged from 0.17 to 0.44. The degree of morphological differentiation within the collection varied depending on trait. Nei's genetic diversity for the combined results for the whole collection was equal to 0.202. Neither unweighted pair group method with arithmetic mean nor principal coordinate analysis showed any discrimination of cultivars according to breeding period and morphological trait. Part of morphological diversity has been preserved in the Polish early oat cultivars, do not exist anymore in contemporary cultivars, and also in landraces. The interest of breeders in early cultivars germplasm stored in genebank was and still is negligible. Breeding is confined to a few preferred by market morphotypes. So, it is very probable that the gene pools of early and contemporary cultivars could be separable. © 2014 The Author(s).