PCR-based DNA Fingerprinting Analysis of Coliphages Isolated from Sewage Polluted Seawater in Alexandria

Abstract

Three coliphage isolates were propagated and purified from sewage polluted water samples. Isolates were selected only on the basis of different plaque size and shape on E. coli host (ATCC 13706). Electron microscopy of purified isolates showed isolate CPS1 to belong to family Siphoviridae whereas isolates CPS2 and CPS3 both belong to family Myoviridae. DNA fingerprinting based on restriction endonucleases analysis showed the three isolates to have no EcoRI, Pst I or Sac I sites. The isolates have different patterns of digestion with Hind III and Sal I enzymes. RAPD-PCR using 10 different primers also indicated that the three isolates have different patterns and could belong to different species of both families