Effect of artificial LED light and far infrared irradiation on phenolic compound, isoflavones and antioxidant capacity in soybean (Glycine max L.) sprout

Abstract

The effect of light emitting diode (LED) light and far infrared irradiation (FIR) on total phenol, isoflavones and antioxidant activity were investigated in soybean (Glycine max L.) sprout. Artificial blue (470 nm), green (530 nm) LED and florescent light (control) were applied on soybean sprout, from three to seven days after sowing (DAS) in growth chamber. The photosynthetic photon flux density (PPFD) and photoperiod was 150 ± 5 µmol m −2 s −1 and 16 h, respectively. The FIR was applied for 30, 60 and 120 min at 90, 110 and 130 ◦ C on harvested sprout. Total phenolic content (TP) (59.81 mg/g), antioxidant capacity (AA: 75%, Ferric Reduction Antioxidant Power (FRAP): 1357 µM Fe 2+ ) and total isoflavones content (TIC) (51.1 mg/g) were higher in blue LED compared to control (38.02 mg/g, 58%, 632 µM Fe 2+ and 30.24 mg/g, respectively). On the other hand, TP (64.23 mg/g), AA (87%), FRAP (1568 µM Fe 2+ ) and TIC (58.98 mg/g) were significantly increased by FIR at 110 ◦ C for 120 min among the treatments. Result suggests that blue LED is the most suitable light to steady accumulation of secondary metabolites (SM) in growing soybean sprout. On the other hand, FIR at 110 ◦ C for 120 min is the best ailment to induce SM in proceed soybean sprout.