Chemical tweaking of a non-fluorescent GFP chromophore to a highly fluorescent coumarinic fluorophore: Application towards photo-uncaging and stem cell imaging

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Abstract

Three step chemical tweaking of nonfluorescent parent GFP chromophore has yielded a three hundred times as bright coumarinic fluorophore. A fluorogenic compound has been prepared which after photo-uncaging yields the same highly bright fluorophore that could be used for live stem cell imaging. © 2013 The Royal Society of Chemistry.

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Chatterjee, T., Roy, D., Das, A., Ghosh, A., Bag, P. P., & Mandal, P. K. (2013). Chemical tweaking of a non-fluorescent GFP chromophore to a highly fluorescent coumarinic fluorophore: Application towards photo-uncaging and stem cell imaging. RSC Advances, 3(46), 24021–24024. https://doi.org/10.1039/c3ra44034f

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