Based on studies performed with a selected mouse monoclonal antitransketolase- like (TKTL)-1 antibody (clone JFC12T10), overexpressionofTKTL-1 has been shown to be correlatedwith poor survival and increased metastatic spread in several human tumor entities. Since the clinical aggressivenessmediated by TKTL-1 has been partially related to resistance to hypoxia,we originally aimed to explore the influence of hypoxia on the expression of TKTL-1. Unexpectedly, results of our experiments indicated that the antibody clone JFC12T10 lacks target specificity. Since the majority of data on the role of TKTL-1 in human cancer is based upon studies performed with this antibody clone, we subsequently re-evaluated the expression of TKTL-1 in six different cancer cell lines (HeLa, MCF-7, A549, HT-1080, M21 and TF-1). Using RT-PCR and consecutive sequence analysis, we show that transketolase (TKT), not TKTL-1, is the dominant isoform of transketolases in the cell lines analyzed. Our data argue against a major role of TKTL-1 for the metabolism of cancer cells. © 2011 Springer Science+Business Media, LLC.
CITATION STYLE
Mayer, A., Von Wallbrunn, A., & Vaupel, P. (2011). Evidence against a major role for TKTL-1 in hypoxic and normoxic cancer cells. In Advances in Experimental Medicine and Biology (Vol. 701, pp. 123–128). Springer New York LLC. https://doi.org/10.1007/978-1-4419-7756-4_17
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