By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.
Blibech, M., Ellouz Ghorbel, R., Chaari, F., Dammak, I., Bhiri, F., Neifar, M., & Ellouz Chaabouni, S. (2011). Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds . ISRN Microbiology, 2011, 1–5. https://doi.org/10.5402/2011/938347