Adenoviral vector (AdV) of the third generation also known as helper-dependent adenoviral vector (HDV) is an attractive delivery system for gene therapy applications. However, obtaining high quality-grade HDV in sufficient amount remains a challenge that hampers the extensive use of this vector in preclinical and clinical studies. Here we review recent progress in the large-scale manufacturing of HDV. The production of HDV is now amenable to large-scale volume with reduced process duration under optimized rescue and co-infection conditions. Also, efficient downstream processing of HDV with acceptable recovery of HDV and minimal contamination by the helper virus is described.
CITATION STYLE
Dormond, E., & Kamen, A. A. (2011). Manufacturing of adenovirus vectors: production and purification of helper dependent adenovirus. Methods in Molecular Biology (Clifton, N.J.). https://doi.org/10.1007/978-1-61779-095-9_6
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