Cryopreservation is the storage of viable bioresources at ultra-low temperatures in liquid nitrogen (LN). This chapter provides an overview of those protocols most commonly used to cryopreserve in vitro derived shoot tips and meristems; they are described generically, as sequential technical steps, including preparative and cryogenic treatments and the morphogenetic assessment of recovery. The importance of translating research-generated methods into formal Standard Operating Procedures (SOPs) is considered.
CITATION STYLE
Benson, E. E., & Harding, K. (2012). Cryopreservation of shoot tips and meristems: An overview of contemporary methodologies. In Methods in Molecular Biology (Vol. 877, pp. 191–226). Humana Press Inc. https://doi.org/10.1007/978-1-61779-818-4_16
Mendeley helps you to discover research relevant for your work.