Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting. © Springer Science+Business Media New York 2013.
CITATION STYLE
Spitzer-Rimon, B., Cna’Ani, A., & Vainstein, A. (2013). Virus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes. Methods in Molecular Biology, 975, 139–148. https://doi.org/10.1007/978-1-62703-278-0_10
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