Anti-inflammatory Activity of Sting Protein from Apis mellifera

Abstract

-The objective of our study is to determine its anti-inflammatory potential of protein extracted from the stings of honey bee (Apis mellifera). In this study, protein extracted from the stings of Apis mellifera using Tris HCl/ice cold acetone and determined through Nano drop method and then determined its Da protein using SDS-PAGE. In addition, indirect ELISA was performed using rubella vaccine as coating antigen and determined its antibody titre using variable concentration of sting protein (15.62-250 µg) and also determined its activity on human whole blood for determining total cellular content and proliferation against rubella vaccine antigen. The results showed that protein from stings of Apis mellifera showed drastic declined in antibody titre at higher doses but there is slightly enhancement in antibody titre, total cellular content and proliferations at lower concentration as compared to control and rubella vaccine (standard).Overall, this study suggest that stings protein of Apis mellifera showed anti-inflammatory potential against rubella vaccine antigen.