Alopecia areata (AA) is a suspected hair follicle specific autoimmune disease. The potential for cell transfer of AA using the C3H/HeJ mouse model was examined. Cells isolated from lymph nodes and spleens of AA-affected mice using magnetic bead conjugated monoclonal antibodies were subcutaneously injected into normal C3H/HeJ recipients. Within 5 wk, all CD8+ cell-injected mice exhibited localized hair loss exclusively at the site of injection that persisted until necropsy. In contrast, some CD4+ and CD4 +/CD25- cell-injected mice developed extensive, systemic AA, and a combination of CD8+ and CD4+/CD25- cells injected yielded the highest frequency of systemic AA induction. CD4 +/CD25+ cells were less able to transfer the disease phenotype, partially blockaded systemic AA induction by CD4+/ CD25- cells, and prevented CD8+ cell-induced, injection site-localized hair loss. CD11c+ and CD19+ cells failed to promote significant phenotype changes. Increases in co-stimulatory ligands CD40 and CD80, plus increased leukocyte apoptosis resistance with reduced CD95, CD95L, and CD120b expression, were associated with successful alopecia induction. The results suggest that CD8+ cells may be the primary instigators of the hair loss phenotype. However, systemic disease expression fate is, apparently determined by CD4+/CD25- cells, while CD4+/CD25+ lymphocytes may play a predominantly regulatory role. Copyright © 2005 by The Society for Investigative Dermatology, Inc.
CITATION STYLE
McElwee, K. J., Freyschmidt-Paul, P., Hoffmann, R., Kissling, S., Hummel, S., Vitacolonna, M., & Zöller, M. (2005). Transfer of CD8+ cells induces localized hair loss whereas CD4+/CD25- cells promote systemic alopecia areata and CD4+/CD25+ cells blockade disease onset in the C3H/HeJ mouse model. Journal of Investigative Dermatology, 124(5), 947–957. https://doi.org/10.1111/j.0022-202X.2005.23692.x
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