Quantitative proteome profiling of microorganisms by isotopic labeling of amino acids is still a challenge, because only microorganisms with auxotrophic character are able to embed amino acids into their biomass in a quantitatively correct manner. Here, we describe an isotopic labeling technique (sulfur stable isotope labeling of amino acids for quantification, SULAQ) for the sulfur-containing amino acids cysteine and methionine in a broad range of organisms. The metabolic labeling approach is suitable for gel-based and gel-free protein analysis.
CITATION STYLE
Herbst, F. A., Jehmlich, N., von Bergen, M., & Schmidt, F. (2018). Sulfur- 34 S and 36 S stable isotope labeling of amino acids for quantification (SULAQ34/36) of proteome analyses. In Methods in Molecular Biology (Vol. 1841, pp. 163–174). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8695-8_12
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