Interleukin-10 promoter polymorphisms in rheumatoid arthritis and Felty's syndrome

Abstract

Objective. To examine whether promoter polymorphisms associated with variation in interleukin-10 (IL-10) production are relevant to the development of rheumatoid arthritis (RA) or Felty's syndrome (FS). Methods. DNA was obtained from 44 FS patients, 117 RA patients and 295 controls. The promoter region between -533 and -1120 was amplified by polymerase chain reaction, and polymorphisms detected by restriction enzyme digest or sequence-specific oligonucleotide probing. Results. We found no significant difference in allele or haplotype frequencies between the groups. Conclusion. There is no association between FS or RA and these recently identified IL-10 promoter polymorphisms. Other genetic or environmental factors could explain the alterations in IL-10 levels seen in these conditions.