In vitro mass clonal propagation of Spathoglottis plicata Blume

Abstract

For in vitro clonal propagation of Spathoglottis plicata Blume nodal segments of young shoots were cultured on half strength of MS with 2% sucrose + 2.0 mg/l BA + 0.5 mg/l NAA + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC, incubated at 24 ± 2oC under 3000 lux fluorescent light for a 16 hr photoperiod per day. About 19 microshoots were induced from the explants within 12 weeks. Subculture of microshoots for eight weeks on the same nutrient medium enhanced the number of micro-shoots up to 60. The clumps of the micro-shoots were dissected and cultured on half strength of MS with 2% sucrose + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC + 200 mg/l L-glutamine. The micro-shoot sections elongated to form shoots, and new micro shoots were induced from the base within eight weeks of culture. For plantlet formation the best rooting medium was determined as half strength of MS with 2% sucrose + 2 g/l peptone + 15% (v/v) CW + 0.5 g/l AC + 50 g/l banana pulp. After rearing 25 g mixture of urea, TSP and MOP (2 : 1 : 1) were applied per plant at three months intervals. All the regenerated plants blossomed on the third year.