T-cell intracellular antigen-1 (TIA-1)-induced translational silencing promotes the decay of selected mRNAs

62Citations
Citations of this article
76Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Gene array analysis revealed that a subset of mRNAs overexpressed in macrophages lacking the destabilizing factor TTP are also overexpressed in macrophages lacking the translational silencer TIA-1. We confirmed that a representative transcript, apobec-1, is significantly stabilized in cells lacking TIA-1. Tethering TIA-1 to a reporter transcript also promotes mRNA decay, suggesting that TIA-1-mediated translational silencing can render mRNA susceptible to the decay machinery. TIA-1-mediated decay is inhibited by small interfering RNAs targeting components of either the 5′-3′ (e.g. DCP2) or the 3′-5′ (e.g. exosome component Rrp46) decay pathways, suggesting that TIA-1 renders mRNA susceptible to both major decay pathways. TIA-1-mediated decay is inhibited by cycloheximide and emetine, drugs that stabilize polysomes, but is unaffected by puromycin, a drug that disassembles polysomes. These results suggest that TIA-1-induced polysome disassembly is required for enhanced mRNA decay and that TIA-1-induced translational silencing promotes the decay of selected mRNAs. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.

Cite

CITATION STYLE

APA

Yamasaki, S., Stoecklin, G., Kedersha, N., Simarro, M., & Anderson, P. (2007). T-cell intracellular antigen-1 (TIA-1)-induced translational silencing promotes the decay of selected mRNAs. Journal of Biological Chemistry, 282(41), 30070–30077. https://doi.org/10.1074/jbc.M706273200

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free