Mapping Connectivity Amongst Interneuronal Components of the Locomotor CPG

Abstract

The basic rhythmic activity characteristic of locomotion in mammals is generated by a neural network, located in the spinal cord, known as the locomotor central pattern generator (CPG). Although a great deal of effort has gone into the study of this neural circuit over the past century, identification and characterization of its component interneurons has proven to be challenging, largely due to their location and distribution. Recent work incorporating a molecular approach has provided a great deal of insight into the genetic identity of interneurons that make up this neural circuit, as well as the specific roles that they play during stepping. Despite this progress we still know relatively little regarding the manner in which these neuronal populations are interconnected. In this article we review the interneuronal populations shown to be involved in locomotor activity, briefly summarize their specific function, and focus on experimental work that provides insight into their synaptic connectivity. Finally, we discuss how recently developed viral approaches can potentially be incorporated to provide further insight into the network structure of this neural circuit.