Regulation of the G-protein regulatory-Gαi signaling complex by nonreceptor guanine nucleotide exchange factors

Abstract

Background: The GPR-Gαi complex has diverse functional roles, but regulatory mechanisms are not defined. Results: The GPR-Gαi complex is regulated by Ric-8A but not by increased expression of AGS1 or GIV/Girdin. Conclusion: The GPR-Gαi complex is differentially regulated by specific guanine nucleotide exchange factors. Significance: The GPR proteins, Gαi1 and Ric-8A, exhibit dynamic interactions in the cell that influence their subcellular localization and regulate complex formation. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.