C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed. © 2014 Springer Science+Business Media, New York.
CITATION STYLE
Mohlin, F. C., & Blom, A. M. (2014). Purification and functional characterization of C4b-binding protein (C4BP). Methods in Molecular Biology, 1100, 169–176. https://doi.org/10.1007/978-1-62703-724-2_14
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