Abstract
WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates. © Springer Science+Business Media New York 2013.
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APA
Ruan, X., & Valvano, M. A. (2013). In vitro O-antigen ligase assay. Methods in Molecular Biology, 1022, 185–197. https://doi.org/10.1007/978-1-62703-465-4_15
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