Wnt/b-catenin signaling plays a critical role in colonic carcinogenesis. However, non-coding RNAs (ncRNA) transcriptionally regulated by b-catenin are largely unknown. Herein, we found that lncRNA MIR100HG (lnc-MIR100HG) negatively correlated with target genes of b-catenin from The Cancer Genome Atlas colorectal carcinoma database, which was verified in 48 paired colorectal carcinoma specimens. In addition, constitutive overexpression of b-catenin decreased primary and mature lnc-MIR100HG levels, whereas blockage of b-catenin activity with siRNA or inhibitors significantly increased their expression. DNA pull-down and chromatin immunoprecipitation revealed the binding of b-catenin/TCF4 to the MIR100HG promoter. Moreover, b-catenin–forced expression reduced the enrichment of H3K27Ac, an active transcription marker, on the promoter, whereas b-catenin inhibition reversed this effect. Furthermore, HDAC6 was recruited to the MIR100HG promoter and downregulated H3K27Ac enrichment in a b-catenin–dependent manner. Besides, HDAC6 was upregulated and negatively correlated with lnc-MIR100HG in colorectal carcinoma specimens. Functional studies showed that lnc-MIR100HG overexpression induced cell-cycle G0–G1 arrest and repressed cell proliferation via p57 upregulation in vitro and in vivo. Taken together, we found that ectopic b-catenin transcriptionally repressed lnc-MIR100HG expression through HDAC6-mediated histone modification in colorectal carcinoma. Lnc-MIR100HG regulates the cell cycle through p57. Implications: It provides a novel downstream mechanism highlighting b-catenin action during colon carcinogenesis and may shed light for further therapeutic approaches.
CITATION STYLE
Peng, J., Ma, Y., Zhao, X., Yang, X., & Wang, H. (2022). Constitutive b-Catenin Overexpression Represses Lncrna MIR100HG Transcription via HDAC6-Mediated Histone Modification in Colorectal Cancer. Molecular Cancer Research, 20(6), 949–959. https://doi.org/10.1158/1541-7786.MCR-21-0923
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