Whole-Body Imaging of Hematopoietic and Cancer Cells Using Near-Infrared Probes

Abstract

Putting fluorescent and other labels on proteins and small molecules in living cells is an important tool for studying cell biology and developing drugs. The required chemical reactions need to be bioorthogonal to produce selectivity. Here, we summarize currently used bioorthogonal reactions that have been successfully applied for labeling biomolecules in cells. Further, we discuss the various methods available to include orthogonally reactive groups into proteins.